Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography
AUTOR(ES)
Sugawara, Eduardo Kinio, Ribeiro Neto, Luciane Maria, Verreschi, Ieda Terezinha do Nascimento
FONTE
Química Nova
DATA DE PUBLICAÇÃO
2010
RESUMO
Determination of free urinary cortisol is a test of choice in the diagnosis of Cushing's syndrome. In this study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide as internal standard (IS). A BDS-Hypersil-C18® column, water-acetonitrile (72:28; v/v), with a flow rate of 1.0 mL/min and detection at 243 nm were used. This method showed to be both effective and efficient, with sensitivity and linearity ranging from 2.50 to 150 μg/L, and can be used in substitution to the radioimmunoassay technique within this concentration range.
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