Determination of lipid A and endotoxin in serum by mass spectroscopy
AUTOR(ES)
Maitra, Shyamal K.
RESUMO
A quantitative technique for determining lipid A content of endotoxin added to serum by combined gas chromatography-mass spectrometry is described. This technique uses detection of the β-hydroxymyristic acid content of Salmonella minnesota R595 lipopolysaccharide by selected ion monitoring at atomic mass unit of 315.4 The fatty acids produced on hydrolysis of serum containing lipopolysaccharide were extracted and the methyl esters were made. Silica gel chromatography was used to separate methyl esters of hydroxy fatty acids from other fatty acid methyl esters. Trimethylsilyl ether derivatives of the hydroxy fatty acid methyl ester fraction were quantitated by this technique. As little as 200 fmol of β-hydroxymyristic acid could be detected.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=392916Documentos Relacionados
- Detecting alterations of glucose and lipid components in human serum by near-infrared Raman spectroscopy
- Determination of molecular order in supported lipid membranes by internal reflection Fourier transform infrared spectroscopy.
- Determination of serum aluminum, platelet aggregation and lipid peroxidation in hemodialyzed patients
- Determination of Moxidectin in Serum by Liquid Chromatography-Tandem Mass Spectrometry and Its Application in Pharmacokinetic Study in Lambs
- Early-phase endotoxin tolerance: induction by a detoxified lipid A derivative, monophosphoryl lipid A.
