Determination of plasmid-associated hydrophobicity of Yersinia enterocolitica by a latex particle agglutination test.
AUTOR(ES)
Lachica, R V
RESUMO
A quick and simple method was developed to distinguish hydrophobic from hydrophilic cells. The latex particle agglutination test is based on the hydrophobic interactions between cells and latex particles which result in the agglutination of the suspension mixture. There was a direct correlation between the expression of plasmid-associated cell surface properties and latex particle agglutination by Yersinia enterocolitica. Multivalent cation-induced agglutination of suspensions of washed cells of virulent Y. enterocolitica and latex particles is indicative of their amphipathic character. Electrostatic interaction may also play a role in the latex particle agglutination reaction.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=271150Documentos Relacionados
- Plasmid-associated cell surface charge and hydrophobicity of Yersinia enterocolitica.
- Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis.
- Virulence plasmid-associated autoagglutination in Yersinia spp.
- Simple Method for Demonstration of Differential Colony Morphology of Plasmid-Associated Virulent Clones of Yersinia enterocolitica
- Complete DNA Sequence of Yersinia enterocolitica Serotype 0:8 Low-Calcium-Response Plasmid Reveals a New Virulence Plasmid-Associated Replicon