Determination of the Molecular Weight of Animal RNA Viral Genomes by Nuclease Digestions I. Vesicular Stomatitis Virus and Its Defective T Particle
AUTOR(ES)
Repik, Patricia
RESUMO
A procedure has been developed for the determination of the weight of animal RNA virus genomes using controlled nuclease digestions and computation of the moles of oligonucleotides obtained from 1 mol of RNA. Using both pancreatic RNase and RNase T1 to digest viral RNA labeled by 3H-uridine, 3H-cytidine, or 3H-guanosine, the weight of the virion RNA of vesicular stomatitis virus (VSV) is estimated as 3.82 ± 0.14 × 106 whereas that of the VSV-defective T particle is estimated as 1.23 ± 0.04 × 106.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=356726Documentos Relacionados
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