Development and validation of spectrophotometric method for the determination of DPP-4 inhibitor, sitagliptin, in its pharmaceutical preparations
AUTOR(ES)
Sekaran, C. Bala, Rani, A. Prameela
FONTE
Eclética Química
DATA DE PUBLICAÇÃO
2010-09
RESUMO
A simple, sensitive and reproducible spectrophotometric method was developed for the determination of sitagliptin phosphate in bulk and in pharmaceutical formulations. The proposed method is based on condensation of the primary amino group of sitagliptin phosphate with acetyl acetone and formaldehyde producing a yellow colored product, which is measured spectrophotometrically at 430nm. The color was stable for about 1 hour. Beer's law is obeyed over a concentration range of 5-25 µg/ml. The apparent molar absorptivity and Sandell sensitivity values are 1.067 x 10(4) Lmol-1cm-1 and 0.0471 µgcm-2 respectively. All the variables were studied to optimize the reaction conditions. No interference was observed in the presence of common pharmaceutical excipients. The validity of the method was tested by analyzing sitagliptin phosphate in its pharmaceutical preparations. Good recoveries were obtained. The developed method was successfully employed for the determination of sitagliptin phosphate in various pharmaceutical preparations.
Documentos Relacionados
- Studies on the development of spectrophotometric method for the determination of haloperidol in pharmaceutical preparations
- Development and validation of UV spectrophotometric method for determination of levofloxacin in pharmaceutical dosage forms
- Development and Validation of a New Spectrophotometric Method for the Determination of Cephalexin Monohydrate in Pure Form and Pharmaceutical Formulations
- A simple spectrophotometric method for the determination of captopril in pharmaceutical preparations using ammonium molybdate
- Development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations