Development of a Dipstick Assay for Detection of Leishmania-Specific Canine Antibodies
AUTOR(ES)
Schallig, Henk D. F. H.
FONTE
American Society for Microbiology
RESUMO
A dipstick assay, based on Leishmania infantum antigen, for the rapid detection of Leishmania-specific antibodies in canine serum samples was developed and evaluated. After determination of optimal dipstick test conditions, test performance was compared with two existing serological tests, i.e., the direct agglutination test (DAT) and the fast agglutination screening test (FAST). In the present study the dipstick test had a sensitivity of 99.2% and a specificity of 87.9%. The DAT had a sensitivity of 97.7% and a specificity of 95.2%, whereas the FAST had also a sensitivity of 97.7% and a specificity of 93.0%. High degrees of agreement were observed between the dipstick test and DAT (93.7%; κ value, 0.86), between the dipstick test and FAST (91.8%; κ value, 0.82), and between the DAT and FAST (95.2%; κ value, 0.90). The high sensitivity and ease of performance make the dipstick test very suitable for surveillance surveys.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=321708Documentos Relacionados
- LEPTO dipstick, a dipstick assay for detection of Leptospira-specific immunoglobulin M antibodies in human sera.
- Comparison of a Dipstick Assay for Detection of Brucella-Specific Immunoglobulin M Antibodies with Other Tests for Serodiagnosis of Human Brucellosis
- Application of a direct agglutination test for detection of specific anti-Leishmania antibodies in the canine reservoir.
- Evaluation of a dipstick enzyme-linked immunosorbent assay for detection of antibodies to dengue virus.
- International Multicenter Evaluation of the Clinical Utility of a Dipstick Assay for Detection of Leptospira-Specific Immunoglobulin M Antibodies in Human Serum Specimens
