Development of a Gene Inactivation System for Bacteroides forsythus: Construction and Characterization of a BspA Mutant

AUTOR(ES)

Honma, Kiyonobu

FONTE

American Society for Microbiology

RESUMO

Bacteroides forsythus is a gram-negative anaerobic bacterium associated with periodontitis. The bspA gene encoding a cell surface associated leucine-rich repeat protein (BspA) involved in adhesion to fibronectin and fibrinogen was recently cloned from this bacterium in our laboratory. We now describe the construction of a BspA-defective mutant of B. forsythus. This is the first report describing the generation of a specific gene knockout mutant of B. forsythus, and this procedure should be useful in establishing the identity of virulence-associated factors in these organisms.

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