Development of a rapid and simple HPLC-UV method for determination of gallic acid in Schinopsis brasiliensis
AUTOR(ES)
Fernandes, Felipe H.A., Batista, Rayanne S. de A., Medeiros, Francinalva D. de, Santos, Fábio S., Medeiros, Ana C.D.
FONTE
Rev. bras. farmacogn.
DATA DE PUBLICAÇÃO
2015-06
RESUMO
AbstractThe aim of this work was to develop and validate an analytical method for the identification of the chemical marker of Schinopsis brasiliensis Engl., Anacardiaceae. It would determine the total polyphenols and flavonoid content by spectrophotometric methodology in the dried extract of plant. The chromatographic profiles of S. brasiliensis were determined using HPLC-UV. The liquid chromatography method was conducted on a Phenomenex Gemini NX C18 column (250 × 4.6 mm, 5 μm). The mobile phase consisted of 0.05% orthophosphoric acid: methanol. The flow rate was 1 ml/min and effluents were monitored at 271 nm. The retention time for gallic acid was 8.5 min. The described method has the advantage of being both rapid and easy. Hence it can be applied for routine quality control analysis of herbal preparation containing S. brasiliensis.
Documentos Relacionados
- Comparison of microbiological assay and HPLC-UV for determination of fluconazole in capsules
- DESENVOLVIMENTO E VALIDAÇÃO DE MÉTODO PARA DETERMINAÇÃO DE ETILENOTIOURÉIA EM URINA EMPREGANDO HPLCUV
- Development and Validation of Ultrasound Assisted and Dispersive Liquid-Liquid Microextractions Combined with HPLC-UV Method for Determination of Bosentan in Human Plasma and Urine
- Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID
- An HPLC-UV method for the measurement of permeability of marker drugs in the Caco-2 cell assay