Developmental modulation of DNA methylation in the fungus Phycomyces blakesleeanus.
AUTOR(ES)
Antequera, F
RESUMO
DNA methylation is a rather sparse event among fungi. Phycomyces blakesleeanus seems to be one of the few exceptions in this context. 5-Methylcytosine represents 2.9% of the total cytosine in spore DNA and is located in approximately the same amount at any of the four CA, CT, CC or CG dinucleotides. A progressive and gradual drop in total 5-methylcytosine parallels the development of the fungus. This demethylation is non random but sequence specific and is not accounted for equally by the four different methylated dinucleotides, CG being much less affected (20% demethylated) than CA, CT and CC (more than 90% demethylated at the same time). "De novo" methylation to restore the initial pattern probably takes place during spore maturation. By using specific hybridization probes we have been able to show that the rRNA genes are not significantly methylated at any stage of development, regardless of their transcription status.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=321976Documentos Relacionados
- Mutations affecting sexual development in Phycomyces blakesleeanus.
- Effect of glucose on isocitrate lyase in Phycomyces blakesleeanus.
- Effect of light on several metabolites of carbohydrate metabolism in Phycomyces blakesleeanus.
- Multilamellar surface layer of the cell wall of Albugo candida and Phycomyces blakesleeanus.
- Nucleotide sequence of the Phycomyces blakesleeanus leu1 gene.
