Diagnosis of parvovirus B19 infection by detection of specific immunoglobulin M antibody in saliva.
AUTOR(ES)
Cubel, R C
RESUMO
Serum and saliva samples were simultaneously collected from patients with B19 infection. Specimens were collected in a period of 1 to 18 days after the onset of symptoms. Saliva samples were collected with a commercial device, OraSure. The quality of these samples was evaluated by determining the concentration of total immunoglobulin G (IgG) by an enzyme immunoassay. The concentration of IgG in these samples ranged from 4.8 to > 250 mg/liter. B19 infection was confirmed for 20 patients by testing sera in a 1: 100 dilution by an IgM capture enzyme immunoassay (MACEIA) and an IgM capture hemadherence test (MACHAT). Saliva samples from these IgM-positive patients were tested neat by MACEIA and MACHAT. IgM could be detected in 11 of 20 (55%) samples by MACEIA and in 15 of 18 (83%) samples by MACHAT. Serum and saliva samples from a further 17 patients with rash were also tested. All of these specimens were unreactive by both assays. These results show that saliva may be a convenient alternative to serum for the diagnosis of recent B19 infection.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=228763Documentos Relacionados
- Acute-Phase-Specific Heptapeptide Epitope for Diagnosis of Parvovirus B19 Infection
- Acute-Phase-Specific Hepatapeptide Epitope for Diagnosis of Parvovirus B19 Infection
- Parvovirus B19 infection in pregnancy.
- Evaluation of serological assays for identification of parvovirus B19 immunoglobulin M.
- Immune response to B19 parvovirus and an antibody defect in persistent viral infection.
