Different core promoters possess distinct regulatory activities in the Drosophila embryo

AUTOR(ES)

Ohtsuki, Sumio

FONTE

Cold Spring Harbor Laboratory Press

RESUMO

There are numerous examples of shared enhancers interacting with just a subset of target promoters. In some cases, specific enhancer–promoter interactions depend on promoter competition, whereby the activation of a preferred target promoter precludes expression of linked genes. Here, we employ a transgenic embryo assay to obtain evidence that promoter selection is influenced by the TATA element. Both the AE1 enhancer from the Drosophila Antennapedia gene complex (ANT-C) and the IAB5 enhancer from the Bithorax complex (BX-C) preferentially activate TATA-containing promoters when challenged with linked TATA-less promoters. In contrast, the rho neuroectoderm enhancer (NEE) does not discriminate between these two classes of promoters. Thus, certain upstream activators, such as Ftz, prefer TATA-containing promoters, whereas other activators, including Dorsal, work equally well on both classes of promoters. These results provide in vivo evidence that different core promoters possess distinct regulatory activities. We discuss the possibility that an invariant TFIID complex can adopt different conformations on the core promoter.

Documentos Relacionados

• Computational analysis of core promoters in the Drosophila genome
• Drosophila Mediator Complex Is Broadly Utilized by Diverse Gene-Specific Transcription Factors at Different Types of Core Promoters
• CtBP-dependent activities of the short-range Giant repressor in the Drosophila embryo
• Compatibility between enhancers and promoters determines the transcriptional specificity of gooseberry and gooseberry neuro in the Drosophila embryo.
• Promoters from genes for plastid proteins possess regions with different sensitivities toward red and blue light.