Different Mechanisms of Energy Coupling for the Active Transport of Proline and Glutamine in Escherichia coli
AUTOR(ES)
Berger, Edward A.
RESUMO
The ability of either glucose or D-lactate to energize active transport of amino acids in E. coli was studied in starved cells blocked at specific sites of energy metabolism. Proline uptake could be driven by either oxidative or substrate-level processes. The oxidative pathway was sensitive to cyanide but not to arsenate, and operated normally in a mutant deficient in the Ca, Mg-dependent ATPase. The substrate-level pathway, which was active with glucose but not with D-lactate as the carbon source, was sensitive to arsenate but not to cyanide, and required a functional ATPase. Uncouplers prevented the utilization of energy for proline uptake by either pathway.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433532Documentos Relacionados
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