Different roles of bases within the integration signal sequence of human immunodeficiency virus type 1 in vitro.
AUTOR(ES)
Yoshinaga, T
RESUMO
To investigate the roles of bases near the tips of each strand of the long terminal repeat of the human immunodeficiency virus type 1 in the integration reaction, we examined the efficiencies of both binding and integration activities of staggered-ended substrates and mismatched mutant substrates by the integration assay and the UV cross-linking assay. Our results suggest that some bases of the human immunodeficiency virus type 1 long terminal repeat are required primarily for binding, whereas others are more critical for later reaction steps in vitro.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=189032Documentos Relacionados
- Integration of human immunodeficiency virus type 1 DNA in vitro.
- Human immunodeficiency virus type 1 nucleocapsid protein specifically stimulates Mg2+-dependent DNA integration in vitro.
- Circularization of human immunodeficiency virus type 1 DNA in vitro.
- Effect of different donor cells on human immunodeficiency virus type 1 replication and selection in vitro.
- Elements upstream of the AAUAAA within the human immunodeficiency virus polyadenylation signal are required for efficient polyadenylation in vitro.
