Different Types of Recombination Events Are Controlled by the Rad1 and Rad52 Genes of Saccharomyces Cerevisiae
AUTOR(ES)
Klein, H. L.
RESUMO
Intrachromosomal recombination within heteroallelic duplications located on chromosomes III and XV of Saccharomyces cerevisae has been examined. Both possible orientations of alleles have been used in each duplication. Three recombinant classes, gene conversions, pop-outs and triplications, were recovered. Some of the recombinant classes were not anticipated from the particular allele orientation of the duplication. Recovery of these unexpected recombinants requires the RAD1 gene. These studies show that RAD1 has a role in recombination between repeated sequences, and that the recombination event is a gene conversion associated with a crossover. These events appear to involve very localized conversion of a heteroduplex region and are distinct from RAD52 mediated gene conversion events. Evidence is also presented to suggest that most recombination events between direct repeats are intrachromatid, not between sister chromatids.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1203516Documentos Relacionados
- Multiple recombination pathways for sister chromatid exchange in Saccharomyces cerevisiae: role of RAD1 and the RAD52 epistasis group genes
- Induction of Ty Recombination in Yeast by Cdna and Transcription: Role of the Rad1 and Rad52 Genes
- Effects of controlled RAD52 expression on repair and recombination in Saccharomyces cerevisiae.
- Two DNA repair and recombination genes in Saccharomyces cerevisiae, RAD52 and RAD54, are induced during meiosis.
- Two different types of double-strand breaks in Saccharomyces cerevisiae are repaired by similar RAD52-independent, nonhomologous recombination events.