Differential translation in normal and adenovirus type 5-infected human cells and cell-free systems.
AUTOR(ES)
Cherney, C S
RESUMO
When uninfected or adenovirus 5-infected KB cells are exposed to hypertonic medium, the incorporation of radioactive amino acids into protein decreases in both, but more severely in the uninfected cells. Although the effect of hypertonic medium on the synthesis of specific polypeptides varies, the translation of viral polypeptides as a class is less inhibited. The same patterns of proteins are synthesized regardless of the solute used in the hypertonic medium. The mechanism by which hypertonic conditions exert their effect on whole cells was investigated in K cell-free systems. It was possible to simulate the differential patterns of protein synthesis obtained in whole cells in hypertonic medium by increasing ion concentrations in cell-free extracts which are capable of initiating polypeptide chains on exogenous templates. However, in cell lysates which only elongate proteins, the same patterns were not obtained. Certain host and viral polypeptides displayed striking responses to increased ionic conditions in whole cells and cell-free systems. The synthesis of a host 44K protein, actin, appeared to be most sensitive; lower-molecular-weight proteins were fairly resistant. Among the viral proteins, the synthesis of 100K was inhibited, but most notable was the marked resistance of the synthesis of polypeptide IX. Possible mechanisms for differential synthesis and their significance are considered.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=353357Documentos Relacionados
- Normal translation of human adenovirus mRNA in cell-free lysates prepared from abortively as well as productively infected monkey cells.
- Context effects and inefficient initiation at non-AUG codons in eucaryotic cell-free translation systems.
- In vitro synthesis and processing of herpes simplex virus type 2 gG-2, using cell-free transcription and translation systems.
- Cell-Free Translation of Highly Purified Adenovirus Messenger RNA
- Translation of murine leukemia virus RNA in cell-free systems from animal cells.
