Differentiation of Aspergillus ustus Strains by Random Amplification of Polymorphic DNA

AUTOR(ES)

Rath, Peter-Michael

FONTE

American Society for Microbiology

RESUMO

The sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns of water-soluble proteins and the randomly amplified polymorphic DNA (RAPD) patterns of whole-cell lysates from 21 Aspergillus ustus isolates, including 11 reference strains and 10 patient and environmental strains from one hospital, were investigated. All isolates showed identical protein patterns. The RAPD assay discriminated between all reference strains. Comparison of hospital isolates showed identical RAPD patterns in some of the patient and environmental isolates. The data indicate that the RAPD technique is useful for fingerprinting A. ustus.

Documentos Relacionados

• Differentiation of Aspergillus parasiticus from Aspergillus sojae by random amplification of polymorphic DNA.
• "Pseudoclusters" and typing by random amplification of polymorphic DNA of Aspergillus fumigatus.
• Application of polymerase chain reaction to fingerprinting Aspergillus fumigatus by random amplification of polymorphic DNA.
• Random amplification of polymorphic DNA of penicillinase-producing Neisseria gonorrhoeae strains.
• Molecular typing by random amplification of polymorphic DNA and M13 southern hybridization of related paired isolates of Aspergillus fumigatus.