Dinucleotides as Products of an Exonucleolytic Activity Associated with the Micrococcus luteus DNA Polymerase*
AUTOR(ES)
Harwood, S. J.
RESUMO
The partially purified DNA polymerase from Micrococcus luteus contains a low level of exonucleolytic activity. The enzyme preparation (1200-fold purified) contains approximately 100 times more polymerase than exonuclease activity. Both single- and double-stranded DNA are degraded at the same rate. The predominant products are dinucleoside diphosphates (d-pXpY); mononucleotides and a trace of trinucleotides are also produced. Each of these products is formed at a constant rate throughout the course of the reaction. The nuclease degrades a DNA chain from the 5′-end. The enzyme preparation contains no detectable endonuclease activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=283091Documentos Relacionados
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