Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers.
AUTOR(ES)
Herman, L M
RESUMO
A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=167343Documentos Relacionados
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