Direct Measurement of the pK Values of an Alkaline Bohr Group in Human Hemoglobin
AUTOR(ES)
Kilmartin, J. V.
RESUMO
Proton nuclear magnetic resonance spectra of normal and des-(his 146β) human hemoglobin in the aromatic resonance region have been compared at different pH values. From these measurements, a pK value for histidine 146β of 7.1 in carbonmonoxyhemoglobin and 8.0 in deoxyhemoglobin has been found. These pK values confirm the proposed role of histidine 146β in the alkaline Bohr effect.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433468Documentos Relacionados
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