Direct visualization of the interrelationship between intramembrane and extracellular structures.
AUTOR(ES)
Wade, J B
RESUMO
The apical surface of the toad urinary bladder is covered by an interconnected mesh of glycocalyx, which appears to attach to the plasma membrane bilayer. To evaluate the interrelationship between these extracellular elements and intramembrane structures, a strategy was devised to produce composite replicas that allow the simultaneous visualization of intramembrane particles by freeze-fracture while the glycocalyx mesh is replicated by rotary shadowing of the extracellular surface after freeze-drying. Evaluation of these composite replicas by electron microscopy reveals that contacts occur between extracellular filamentous elements and intramembrane particles. This structural organization may be important for stabilizing intramembrane components and for anchoring extracellular elements to the membrane.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=286990Documentos Relacionados
- Three-dimensional electron microscopical visualization of the cytoskeleton of animal cells: immunoferritin identification of actin- and tubulin-containing structures.
- Extracellular protons acidify osteoclasts, reduce cytosolic calcium, and promote expression of cell-matrix attachment structures.
- Disagreement between calorimetric and van't Hoff enthalpies of assembly of protein supramolecular structures.
- Early stages in Bacillus subtilis transformation: association between homologous DNA and surface structures.
- Self-assembly of biological structures.