Direction of Travel of RecBc Recombinase through Bacteriophage Lambda DNA
AUTOR(ES)
Stahl, Franklin W.
RESUMO
We examined linkage relationships for RecBC-mediated recombination in lytic cycle crosses of λ phages bearing two cohesive end sites ( cos) oriented in the same direction. The relationships obtained imply that a given recombinant tends to be packaged from the cos site that is the nearer one to the right of the exchange. In view of the previously established coupling of entry of a recombinase at a cos cut and initiation of DNA packaging by that cos cut, these results imply that the recombinase (presumably the RecBC gene product) enters λ's chromosome at the right end.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1202835Documentos Relacionados
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