Discrimination of heterogenous mRNAs encoding strychnine-sensitive glycine receptors in Xenopus oocytes by antisense oligonucleotides.

AUTOR(ES)

Akagi, H

RESUMO

Three synthetic oligodeoxynucleotides complementary to different parts of an RNA encoding a glycine receptor subunit were used to discriminate heterogenous mRNAs coding for glycine receptors in adult and neonatal rat spinal cord. Injection of the three antisense oligonucleotides into Xenopus oocytes specifically inhibited the expression of glycine receptors by adult spinal cord mRNA. In contrast, the antisense oligonucleotides were much less potent in inhibiting the expression of glycine receptors encoded by neonatal spinal cord mRNA. Northern blot analysis revealed that the oligonucleotides hybridized mostly to an adult cord transcript of approximately 10 kilobases in size. This band was also present in neonatal spinal cord mRNA but its density was about one-fourth of the adult cord message. There was no intense band in the low molecular weight position (approximately 2 kilobases), the existence of which was expected from electrophysiological studies with size-fractionated mRNA of neonatal spinal cord. Our results suggest that in the rat spinal cord there are at least three different types of mRNAs encoding functional strychnine-sensitive glycine receptors.

Documentos Relacionados

• Extrinsic factors regulate partial agonist efficacy of strychnine-sensitive glycine receptors
• Strychnine-sensitive glycine responses of neonatal rat hippocampal neurones.
• Characteristics of glycine receptors expressed by embryonic rat brain mRNAs
• Release of acetylcholine by Xenopus oocytes injected with mRNAs from cholinergic neurons.
• The maternal store of zinc finger protein encoding mRNAs in fully grown Xenopus oocytes is not required for early embryogenesis.