Dissection of functional domains of adenovirus DNA polymerase by linker-insertion mutagenesis.
AUTOR(ES)
Chen, M
RESUMO
Linker-insertion mutations were introduced into the cloned adenovirus DNA polymerase gene and the functional effects on the initiation and elongation of DNA in vitro were measured. Essential regions of the polymerase appear to be scattered in patches across the entire molecule and are not limited to the five regions of homology shared with a variety of other replicating polymerases. Thus, the adenovirus DNA polymerase presumably contains active sites that must be formed by distant interactions across the polymerase molecule.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=297786Documentos Relacionados
- Identification of functional regions of herpes simplex virus glycoprotein gD by using linker-insertion mutagenesis.
- Analysis of the adenovirus type 5 terminal protein precursor and DNA polymerase by linker insertion mutagenesis.
- Dissection of functional domains of the yeast proton-pumping ATPase by directed mutagenesis.
- Analysis of functional domains of the v-fms-encoded protein of Susan McDonough strain feline sarcoma virus by linker insertion mutagenesis.
- Linker-insertion nonsense and restriction-site deletion mutations of the gB glycoprotein gene of herpes simplex virus type 1.