Dissociation of Cellular Functions in Bacillus cereus by 5-Fluorouracil
AUTOR(ES)
Reich, Melvin
RESUMO
Reich, Melvin (The George Washington University School of Medicine, Washington, D.C.), and H. George Mandel. Dissociation of cellular functions in Bacillus cereus by 5-fluorouracil. J. Bacteriol. 91:517–523. 1966.—5-Fluorouracil (FU) produced a marked inhibition of growth and deoxyribonucleic acid (DNA) synthesis in Bacillus cereus 569H. Protein and ribonucleic acid (RNA) synthesis were not specifically inhibited, and proceeded at the rate of turbidometric increase of the cells. Cell-wall synthesis, respiration, and penicillinase production continued in the presence of FU at essentially the control rate. The addition of equimolar concentrations of uracil and FU prevented growth inhibition but did not restore DNA synthesis. The addition of thymidine with FU did not relieve growth inhibition but did restore the DNA content to normal. Thymidine supplementation also increased the quantity of FU, but not uracil, incorporated into RNA and the acid-soluble fraction. The data indicate that inhibition of growth can be dissociated from inhibition of DNA synthesis and that more DNA is present in normal cells than is needed for growth and reproduction.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=314890Documentos Relacionados
- COMPARATIVE EFFECTS OF 5-FLUOROURACIL ON STRAINS OF BACILLUS MEGATERIUM
- Inhibition of Photoperiodic Induction by 5-Fluorouracil 12
- 5-fluorouracil and beyond.
- Partial Satisfaction of a Uracil Requirement by 5-Fluorouracil
- USE OF 5-FLUOROURACIL FOR THE ISOLATION OF AUXOTROPHIC MUTANTS OF BACILLUS MEGATERIUM