Dissociation of Mammalian Polyribosomes into Subunits by Puromycin
AUTOR(ES)
Blobel, Günter
RESUMO
Hepatic ribosomes have been dissociated into biologically active subunits as follows. Polysomes were treated at 0°C with puromycin at high ionic strength. This released most of the nascent polypeptide chains without dissociating the polysomes, which retained the mRNA and the tRNA moiety of peptidyl tRNA, but were unable to continue the translation of mRNA. The polysomes were then heated to 37°C, when they dissociated completely into subunits. Similar treatment without puromycin resulted in only partial dissociation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388945Documentos Relacionados
- Reversible dissociation of tryptophanase into protein subunits.
- DISSOCIATION OF RABBIT GAMMA GLOBULIN INTO SUBUNITS BY REDUCTION AND ACIDIFICATION*
- Effect of Puromycin Analogues and Other Agents on Peptidyl-Puromycin Synthesis on Polyribosomes
- Peptidyl-Puromycin Synthesis on Polyribosomes from Escherichia coli*
- INHIBITION BY PUROMYCIN OF AMINO ACID INCORPORATION INTO PROTEIN*
