Distribution Pattern of Cell Division Centers on the Epidermis of Stem Segments of Torenia fournieri during de Novo Bud Formation

AUTOR(ES)

Chlyah, Hassane

RESUMO

The stem epidermis in Torenia fournieri, which has budding potentialialities, is composed of one cell layer which can be easily separated from the rest of the stem segment at different stages of bud formation. As the buds are formed directly from the epidermis, without intermediate callus formation, it is possible to observe simultaneously the cell division centers over the entire excised epidermal surface. The quantitative analysis at the 6-day stage of bud formation showed that the cell division centers do not have a random distribution on the epidermal surface. With respect to the length of the stem segment, the frequency of cell division centers increases toward the base which is also the direction of auxin transport. With respect to the width, the maximum number of division centers is observed on either side of the median zone. The median zone and the lateral zones have few division centers. An anatomical study showed that the zones with few division centers are the closest to underlying vascular tissue. A more uniform distribution of division centers can be obtained by addition of auxin to the medium.

Documentos Relacionados

• Inter-Tissue Correlations in Organ Fragments: Organogenetic Capacity of Tissues Excised from Stem Segments of Torenia fournieri Lind Cultured Separately in Vitro
• Guidance in vitro of the pollen tube to the naked embryo sac of torenia fournieri
• CELL DIVISION AND THE DIFFERENTIATION OF WOUND-VESSEL MEMBERS IN CULTURED STEM SEGMENTS OF Coleus*
• Fine-structure evidence for cell membrane partitioning of the nucleoid and cytoplasm during bud formation in Hyphomonas species.
• Dynamics of phragmoplastin in living cells during cell plate formation and uncoupling of cell elongation from the plane of cell division.