DNA-dependent divalent cation binding in the nucleosome core particle

AUTOR(ES)

Davey, Curt A.

FONTE

National Academy of Sciences

RESUMO

Sequence-specific binding of divalent cations to nucleosomal DNA can potentially influence nucleosome position and mobility, as well as modulate interactions with nuclear factors. We define the bonding and specificity of divalent cation interaction with nucleosomal DNA by characterizing Mn2+ binding in the x-ray structure of the nucleosome core particle at 1.9-Å resolution. Manganese ions are found ligated with high occupancy in the major groove to 12 of 22 GG and GC base pair steps. The specific location and mode of metal binding is the consequence of unambiguous conformational differences between dinucleotide sites, owing to their sequence context and orientation in the nucleosome core.

Documentos Relacionados

• DNA-dependent protein kinase: DNA binding and activation in the absence of Ku
• DNA motions in the nucleosome core particle.
• Poxvirus DNA-dependent RNA polymerase.
• Initiation by the DNA-dependent RNA polymerase.
• Oxytricha telomere-binding protein: DNA-dependent dimerization of the alpha and beta subunits.