DNA element of Corynebacterium diphtheriae with properties of an insertion sequence and usefulness for epidemiological studies.
AUTOR(ES)
Rappuoli, R
RESUMO
The segment of DNA which is inserted within the tox gene of bacteriophage gamma and is responsible for its Tox- phenotype was found to be present and repeated approximately 30 times in the chromosome of Corynebacterium diphtheriae Belfanti 1030. Other C. diphtheriae strains contained a variable number of copies (1 to 25) of the same element. Sequence analysis showed that this repeated and interspersed DNA element was flanked by 9-base-pair direct repeats and that the 5' and 3' ends of the insertion contained sequences forming an imperfect inverted repeat. Therefore, the DNA segment here described has most of the typical structural features of a bacterial insertion sequence element. We show that different C. diphtheriae isolates derived from the same outbreak of diphtheria have an identical genomic distribution of this DNA element and that such DNA can be useful for epidemiological studies.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=211769Documentos Relacionados
- Ribotyping of Pseudomonas aeruginosa: discriminatory power and usefulness as a tool for epidemiological studies.
- Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae.
- Definition of hip osteoarthritis for epidemiological studies.
- STUDIES OF MONO- AND POLYLYSOGENIC CORYNEBACTERIUM DIPHTHERIAE1
- THE PROPERTIES OF FORMALDEHYDE-RESISTANT CORYNEBACTERIUM DIPHTHERIAE
