DNA hybridization analysis of the nif region of two methylotrophs and molecular cloning of nif-specific DNA.
AUTOR(ES)
Toukdarian, A E
RESUMO
DNA isolated from two diazotrophic methylotrophs, the obligate methanotroph Methylosinus sp. strain 6 and the methanol autotroph Xanthobacter sp. H4-14, hybridized to DNA fragments encoding nitrogen fixation (nif) genes from Klebsiella pneumoniae. This interspecific nif homology was limited to DNA fragments encoding the nitrogenase structural proteins (nifH, nifD, and nifK) and specific methylotroph DNA sequences. The hybridization patterns obtained with the two methylotrophs were dissimilar, indicating that the nif region of methylotrophs is not physically conserved. By using the K. pneumoniae nif structural genes as a probe, a fragment of nif DNA from each methylotroph was cloned and characterized. The DNA fragment from Methylosinus sp. 6 encoded two polypeptides of 57,000 and 34,000 molecular weight.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=215347Documentos Relacionados
- Purification and properties of a nif-specific flavodoxin from the photosynthetic bacterium Rhodobacter capsulatus.
- Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of mec-specific DNA.
- Molecular cloning of nif DNA from Azotobacter vinelandii.
- Molecular cloning and physical mapping of murine cytomegalovirus DNA.
- Molecular cloning, genetic characterization and DNA sequence analysis of the recM region of Bacillus subtilis.
