DNA-mediated gene transfer of beta-aspartylhydroxamate resistance into Chinese hamster ovary cells.
AUTOR(ES)
Andrulis, I L
RESUMO
Cell lines that have high levels of resistance to beta-aspartylhydroxamate and elevated levels of asparagine synthetase activity were selected in two steps from Chinese hamster ovary cells. Resistance to beta-aspartylhydroxmate was transferred into sensitive cells by using total genomic DNA derived from the dominant two-step mutants. The surviving colonies were characterized as transferants on the basis of transfer frequency, degree of resistance to beta-aspartylhydroxamate, increased level of asparagine synthetase activity, expression of the donor form of asparagine synthetase, codominance in hybrids, and instability of the phenotype in the absence of selection.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=348842Documentos Relacionados
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