DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis
AUTOR(ES)
Soppe, Wim J.J.
FONTE
Oxford University Press
RESUMO
We propose a model for heterochromatin assembly that links DNA methylation with histone methylation and DNA replication. The hypomethylated Arabidopsis mutants ddm1 and met1 were used to investigate the relationship between DNA methylation and chromatin organization. Both mutants show a reduction of heterochromatin due to dispersion of pericentromeric low-copy sequences away from heterochromatic chromocenters. DDM1 and MET1 control heterochromatin assembly at chromocenters by their influence on DNA maintenance (CpG) methylation and subsequent methylation of histone H3 lysine 9. In addition, DDM1 is required for deacetylation of histone H4 lysine 16. Analysis of F1 hybrids between wild-type and hypomethylated mutants revealed that DNA methylation is epigenetically inherited and represents the genomic imprint that is required to maintain pericentromeric heterochromatin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=136960Documentos Relacionados
- Histone H3 lysine 9 methylation is required for DNA elimination in developing macronuclei in Tetrahymena
- Erasure of CpG methylation in Arabidopsis alters patterns of histone H3 methylation in heterochromatin
- Parent-Specific Complementary Patterns of Histone H3 Lysine 9 and H3 Lysine 4 Methylation at the Prader-Willi Syndrome Imprinting Center
- Chromatin immunoprecipitation microarrays for identification of genes silenced by histone H3 lysine 9 methylation
- G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis