DNA packaging initiation of Salmonella bacteriophage P22: determination of cut sites within the DNA sequence coding for gene 3.

AUTOR(ES)

Backhaus, H

RESUMO

DNA packaging of Salmonella phage P22 starts at a defined site on a concatemer of P22 genomes. The molecular ends formed at the packaging initiation site (pac) map within a region of ca. 120 base pairs and may contain any of the four nucleotides at their 5' end. The determination of the positions of the cuts within the sequence demonstrates a characteristic distribution of cut sites which apparently cannot be attributed to the sequence organization of the involved regions. Symmetric elements of the sequence might serve as signals for a recognition event(s) at pac in a separate process preceding the cutting reaction. The region of packaging initiation is located within the sequence coding for gene 3. The 3 protein is responsible for the site specificity of this process. We find no significant homology to Nu1 protein, which appears to have an analogous or similar function in the DNA maturation of Escherichia coli phage lambda.

Documentos Relacionados

• On the sequential packaging of bacteriophage P22 DNA.
• Sequence of the Genome of Salmonella Bacteriophage P22
• Delayed Lysis with Salmonella Bacteriophage P22: Induction of Lysis by Addition of Cysteine or Histidine to the Growth Medium
• The superinfection exclusion gene (sieA) of bacteriophage P22: identification and overexpression of the gene and localization of the gene product.
• Sequence of a DNA injection gene from Salmonella typhimurium phage P22.