DNA regions essential for the function of a bacteriophage fd promoter.
AUTOR(ES)
Okamoto, T
RESUMO
The promoter for the major coat protein gene of bacteriophage fd contains a unique sequence. TATAAT, in the non-transcribed region corresponding to the Pribnow box. A R-Hha I cleavage site which destroys functions is located five pairs upstream from the TATAAT sequence (fifteen base pairs upstream from the RNA initiation site). The promoter was cleaved into two fragments by R-Hha I and each promoter fragment was joined to DNA fragments derived from other regions. Ligation of the TATAAT-containing fragment to any of the DNA fragments examined resulted in recovery of promoter function. The results suggest for this type of promoter that no unique sequence is necessary upstream from the R-Hha I cleavage site although a contiguous DNA chain must be present in this area.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342560Documentos Relacionados
- Multiple DNA sequence elements are necessary for the function of an immunoglobulin heavy chain promoter.
- Nucleotide sequence of bacteriophage fd DNA.
- Studies on bacteriophage fd DNA. III. Nucleotide sequence preceding the RNA start-site on a promoter-containing fragment.
- A thyroid-specific nuclear protein essential for tissue-specific expression of the thyroglobulin promoter.
- A purified transcription factor (TIF-IB) binds to essential sequences of the mouse rDNA promoter.