DNA-RNA hybrid duplexes containing oligo(dA:rU) sequences are exceptionally unstable and may facilitate termination of transcription.
AUTOR(ES)
Martin, F H
RESUMO
A DNA-RNA hybrid oligonucleotide duplex, dC(pA) 5pG:rC(pU)5pG, which contains a (dA:rU) 5 sequence, is at least 200 times less stable at room temperature than the corresponding duplex containing an (rA:dT) 5 sequence, rC(pA)5pG:dC(pT)5pG. This result provides an explanation for the finding that most primary RNA transcripts terminate in several consecutive rU residues, but not rA residues. It strongly supports the idea that instability of the DNA-RNA hybrid at the growing point of transcription plays a role in termination of transcription.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=324078Documentos Relacionados
- CD of homopolymer DNA-RNA hybrid duplexes and triplexes containing A-T or A-U base pairs.
- Participation of a DNA-RNA hybrid complex in in vivo genetic transcription.
- A DNA-RNA complex as an intermediate of in vitro genetic transcription.
- The structure of a DNA-RNA hybrid.
- Theoretical studies of DNA-RNA hybrid conformations.