Dolichyldiphosphoryloligosaccharide—protein oligosaccharyltransferase: Solubilization, purification, and properties
AUTOR(ES)
Das, Rathindra C.
RESUMO
Dolichyldiphosphoryloligosaccharide—protein oligosaccharyltransferase was solubilized from hen oviduct rough endoplasmic reticulum by extraction with 0.2% Nonidet P40. Oligosaccharyltransferase activity was assayed in an incubation mixture containing Glcn-Manx-GlcNAc2-diphosphoryldolichol as an oligosaccharyl donor and the 125I-labeled tryptic peptide consisting of residues 29-58 from bovine α-lactalbumin as acceptor. The transferase was purified approximately 2000-fold by fractionation on a bovine α-lactalbumin-Sepharose column; the active material bound quantitatively to the gel and was eluted by removal of divalent cation from the wash buffer. The product of the transferase activity, 125I-glycopeptide, was determined as concanavalin A-agarose-adsorbed radioactivity by a filter disc assay method. 125I-Labeled concanavalin A-agarose-bound product was characterized as a glycopeptide as follows: (i) gel filtration behavior on Sephadex G-50; (ii) elution from concanavalin A-agarose with 1% α-methyl mannoside; (iii) absence of affinity for ricin-Sepharose and loss of affinity for concanavalin A-agarose after treatment with endo-β-N-acetylglucosaminidase H; (iv) enzymatic synthesis of identical product upon using [3H]oligosaccharyldiphosphoryldolichol and unlabeled peptide acceptor; and (v) digestion of 3H-labeled peptide with Pronase, resulting in the formation of lower molecular weight glycopeptide. Oligosaccharyltransferase activity exhibited an absolute requirement for divalent cations (3 mM Mn2+; Mg2+ was 30% as effective), complete dependence on exogenously supplied peptide acceptor (1.33 μg/ml) and oligosaccharyldiphosphoryldolichol (approximately 10 nmol/ml), and an optimum pH between 7 and 7.5.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=349716Documentos Relacionados
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