Dose Responsiveness and Variation Among Inbred Strains of Mice in Production of Interferon After Treatment with Poly (I)· Poly (C) [Poly-d-Lysine] Complexes

AUTOR(ES)

Rice, J. M.

RESUMO

Poly-d-lysine forms a stochiometric complex with poly(I) · poly(C) which has a higher Tm (83 C in 0.15 m NaCl) than the uncomplexed double-stranded polyribo-nucleotide (63 C). The complex was superior to poly(I) · poly(C) alone as an interferon inducer in vivo. Significant serum interferon titers were attained in Swiss mice during a 24-hr period after intraperitoneal injection of 10 to 300 μg of poly(I) · poly(C) [1.0 poly-d-lysine] complex, at concentrations of 100 to 1,000 μg/ml. The serum interferon responses (average and maximum titers) of a series of inbred strains of mice to a single intraperitoneal injection of 100 μg of complex decreased in the order: Swiss > DBA/2 > C3H > BALB/c > CF-1 > AKR, C57Bl/6, NZB > SJL > NZW and varied by a factor of approximately 100 from the most to the least responsive.

Documentos Relacionados

• Enhancement by Poly-d-Lysine of Poly I: C-Induced Interferon Production in Mice
• Inhibition of bactericidal and bacteriolytic activities of poly-D-lysine and lysozyme by chitotriose and ferric iron.
• Interferon induction by and toxicity of polyriboinosinic acid [poly(rI)].polyribocytidylic acid [poly (rC)], mismatched analog poly (rI).poly[r(C12Uracil)n], and poly(rI).poly(rC) L-lysine complexed with carboxymethylcellulose.
• Transitions of poly(dI-dC), poly(dI-methyl5dC) and poly(dI-bromo5dC) among and within the B-, Z-, A- and X-DNA families of conformations.
• Biologic effects after a single dose of poly(I):poly(C12U) in healthy volunteers.