Double-Bond Requirement in a Fatty Acid Desaturase Mutant of Saccharomyces cerevisiae
AUTOR(ES)
Wisnieski, Bernadine J.
RESUMO
A fatty acid Δ9-desaturase mutant of yeast was analyzed to establish the specificity of the fatty acid structural requirement. Several double-bond-containing and substituted fatty acids were tested. It was concluded that the presence of a Δ9cis double bond is necessary for growth. The need for a specific chain length or a specific number of double bonds, or for both, is more flexible. Tracer-containing 16:1Δ9cis, 18:1Δ9cis, 18:2Δ9,12cis,cis and 18:3Δ9,12,15 all cis revealed that each of these growth-supporting components, once taken up, was not converted into other fatty acids. Concentration effects on doubling time were also considered.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=250465Documentos Relacionados
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