Drosophila RNase Z processes mitochondrial and nuclear pre-tRNA 3′ ends in vivo
AUTOR(ES)
Dubrovsky, Edward B.
FONTE
Oxford University Press
RESUMO
Although correct tRNA 3′ ends are crucial for protein biosynthesis, generation of mature tRNA 3′ ends in eukaryotes is poorly understood and has so far only been investigated in vitro. We report here for the first time that eukaryotic tRNA 3′ end maturation is catalysed by the endonuclease RNase Z in vivo. Silencing of the JhI-1 gene (RNase Z homolog) in vivo with RNAi in Drosophila S2 cultured cells causes accumulation of nuclear and mitochondrial pre-tRNAs, suggesting that JhI-1 encodes both forms of the tRNA 3′ endonuclease RNase Z, and establishing its biological role in endonucleolytic tRNA 3′ end processing. In addition our data show that in vivo 5′ processing of nuclear and mitochondrial pre-tRNAs occurs before 3′ processing.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=373292Documentos Relacionados
- In vivo pre-tRNA processing in Saccharomyces cerevisiae.
- Distribution of both lengths and 5' terminal nucleotides of mammalian pre-tRNA 3' trailers reflects properties of 3' processing endoribonuclease.
- Non-enzymatic excision of pre-tRNA introns?
- Yeast nucleoporin mutants are defective in pre-tRNA splicing.
- Accumulation of pre-tRNA splicing '2/3' intermediates in a Saccharomyces cerevisiae mutant.
