Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples

AUTOR(ES)

Marinheiro, Juliana Cristina, Sanalios, Roberta Braga, Santos, Daniela Carvalho dos, Costa, Cristovão Alves da, Hársi, Charlotte Marianna

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2009-02

RESUMO

Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.

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