Dye-coupled electrode system for the rapid determination of cell populations in polluted water.
AUTOR(ES)
Nishikawa, S
RESUMO
We determined cell populations in polluted waters by using a fuel cell-type electrode. The electrode was constructed from a platinum anode, a silver peroxide cathode, and a membrane filter for retaining microorganisms. The principle of cell number determination is based on sensing a redox dye reduced by the microorganisms with the electrode. Sample solutions containing microorganisms were membrane filtered, and the resulting filter containing microbial cells was attached to the surface of a platinum anode. The electrode was immersed in phosphate buffer solution (0.05 M, pH 7) containing a redox dye (2,4-dichlorophenol-indophenol), and the current generated was measured. The response time of the electrode system was 10 to 20 min, and the current generated was proportional to cell populations above 10(4) cells/ml.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=241924Documentos Relacionados
- Electrode system for determination of microbial cell populations in polluted water.
- Electrode System for the Determination of Microbial Populations
- Fluorometric determination of the DNA concentration in municipal drinking water.
- Degradation of the Dye Reactive Blue 4 by Coupled Photoassisted Electrochemistry at DSA®-Type Electrode
- Role of sublethal injury in decline of bacterial populations in lake water.
