Early effect of interferon on mouse leukemia cells cultivated in a chemostat.
AUTOR(ES)
Tovey, M
RESUMO
Mouse interferon preparations inhibited the multiplication of mouse leukemia L 1210 cells cultivated under steady-state conditions in a chemostat. The use of this sensitive and controlled system led to the detection of a rapid inhibition in the incorporation of (3-H)thymidine into cellular acid-precipitable material 2 hr after the addition of interferon, whereas an effect on cell multiplication was not detected until 22 hr later. Interferon exerted only a transitory effect on the incorporation of (3-H)uridine into acid-precipitable material and no effect on the incorporation of 14-C-amino acids into cellular protein. It is suggested that the chemostat offers many advantages for the investigation of those physiologic factors or chemotherapeutic substances that modify cell division.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=432738Documentos Relacionados
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