Early Intermediate State of Transforming Deoxyribonucleic Acid During Uptake by Bacillus subtilis1

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RESUMO

Examination of the effects of ethylenediaminetetraacetic acid (EDTA) on uptake of transforming deoxyribonucleic acid (DNA) by Bacillus subtilis allowed definition of a new intermediate state of transforming DNA during uptake. Markers in this state, termed “EDTA-resistant,” are bound to the cell, are sensitive to inactivation by deoxyribonuclease, but may become deoxyribonuclease-resistant despite the addition of excess EDTA. Markers become EDTA-resistant quadratically with time after the addition of DNA to a competent culture, but linearly after exposure of competent cells to a brief pulse of DNA. An attractive model consistent with these findings is that EDTA blocks only the initiation of entry of a molecule of DNA.

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