Early steps in the assembly of vesicular stomatitis virus nucleocapsids in infected cells.
AUTOR(ES)
Dillon, P J
RESUMO
The assembly of nucleocapsids is an essential step in the replicative cycle of vesicular stomatitis virus (VSV). In this study, we have examined the early events of vesicular stomatitis virus nucleocapsid assembly in BHK-21 cells. Nuclease-resistant intracellular nucleocapsids were isolated at various stages of assembly and analyzed for RNA and protein contents. The smallest ribonucleoprotein complex formed during nucleocapsid assembly contains the 5'-terminal 65 nucleotides of nascent viral RNA complexed with the viral proteins N and NS. Elongation of the assembling nucleocapsids proceeds unidirectionally towards the 3' terminus by the sequential addition of viral proteins which incrementally protect short stretches of the growing RNA chain. Pulse-chase studies show that the assembling nucleocapsids can be chased into full-length nucleocapsids which are incorporated into mature virions. Our results also suggest an involvement of the cytoskeletal framework during nucleocapsid assembly.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=253185Documentos Relacionados
- Replicative RNA synthesis and nucleocapsid assembly in vesicular stomatitis virus-infected permeable cells.
- Assembly of vesicular stomatitis virus: distribution of the glycoprotein on the surface of infected cells.
- Assembly and transcription of synthetic vesicular stomatitis virus nucleocapsids.
- Assembly of vesicular stomatitis virus nucleocapsids in vivo: a kinetic analysis.
- Cell-free synthesis and assembly of vesicular stomatitis virus nucleocapsids.