EBV-inducing factor from platelets exhibits growth-promoting activity for NIH 3T3 cells.
AUTOR(ES)
Bauer, G
RESUMO
An Epstein-Barr virus-indicating factor (EIF) has been purified from serum and platelets. We show here that highly purified preparations of platelet EIF exhibit growth-promoting activity for NIH 3T3 cells maintained in platelet-poor plasma. The Epstein-Barr virus (EBV)-inducing activity and growth-promoting activity co-elute upon gel chromatography under non-dissociating as well as dissociating conditions and co-migrate in SDS-gel electrophoresis, supporting the notion that both activities reside on the same molecule. Furthermore, both activities require a pH shock for full activity and act in the same concentration range. The growth-promoting activity of EIF can be differentiated from that of platelet-derived growth factor (PDGF), biologically (on the basis of differential response of cell lines to both factors), biochemically (on the basis of differences in isoelectric points and mol. wts. and the requirement of EIF to become activated by a pH shock) and by the lack of inhibition of EIF by antibody to PDGF.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=554447Documentos Relacionados
- Reconstituted human granulocyte-macrophage colony-stimulating factor receptor transduces growth-promoting signals in mouse NIH 3T3 cells: comparison with signalling in BA/F3 pro-B cells.
- Serum stimulation of phospholipase A2 and prostaglandin release in 3T3 cells is associated with platelet-derived growth-promoting activity.
- Heat shock induces the release of fibroblast growth factor 1 from NIH 3T3 cells.
- Heparin-binding growth factor 1 stimulates tyrosine phosphorylation in NIH 3T3 cells.
- Purification and characterization of a human T-lymphocyte-derived glial growth-promoting factor.
