Ectopic recombination within homologous immunoglobulin mu gene constant regions in a mouse hybridoma cell line.

AUTOR(ES)

Baker, M D

RESUMO

We have transferred a pSV2neo vector containing the wild-type constant region of the immunoglobulin mu gene (C mu) into the mutant hybridoma igm482, which bears a 2-bp deletion in the third constant-region exon of its haploid chromosomal mu gene (C mu 3). Independent igm482 transformants contain the wild-type immunoglobulin C mu region stably integrated in ectopic chromosomal positions. We report here that the wild-type immunoglobulin C mu region can function as the donor sequence in a gene conversion event which corrects the 2-bp deletion in the mutant igm482 chromosomal C mu 3 exon. The homologous recombination event restores normal immunoglobulin M production in the mutant cell.

Documentos Relacionados

• Homologous recombination can restore normal immunoglobulin production in a mutant hybridoma cell line.
• High-frequency homologous recombination between duplicate chromosomal immunoglobulin mu heavy-chain constant regions.
• Regulated expression of an immunoglobulin kappa gene introduced into a mouse lymphoid cell line.
• Stimulation of kappa light-chain gene rearrangement by the immunoglobulin mu heavy chain in a pre-B-cell line.
• Expression of mu and gamma immunoglobulin heavy chains in different cells of a cloned mouse lymphoid line.