Editing of a tRNA anticodon in marsupial mitochondria changes its codon recognition.
AUTOR(ES)
Janke, A
RESUMO
RNA editing has been described for organellar genes of protozoa and plants as well as in three cases for nuclear transcripts of mammals. The mitochondrial tRNA for aspartic acid is encoded in the mitochondrial genome of marsupials with the anticodon GCC. However, cDNA from the gene product revealed that the second nucleotide of the anticodon of the tRNA is posttranscriptionally changed to be recognized as an uridine residue. This restores the coding property of the tRNA. Direct sequencing as well as clones of the amplified cDNA indicated that about 50% of the cellular pool of pre-tRNA molecules for aspartic acid is edited. Thus, RNA editing occurs in mammalian mitochondria and can affect the anticodons of tRNAs.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=309357Documentos Relacionados
- Anticodon-anticodon interaction induces conformational changes in tRNA: yeast tRNAAsp, a model for tRNA-mRNA recognition.
- On the mechanism of tRNA methylase-tRNA recognition.
- Rabbit liver tRNA1Val:I. Primary structure and unusual codon recognition.
- A major lysine tRNA with a CUU anticodon in insect mitochondria.
- Molecular mechanism of codon recognition by tRNA species with modified uridine in the first position of the anticodon.