Efeito dos extratos de Harpagohytum procumbens (garra-do-diabo) e suas frações na atividade da COX-1 e COX-2 e na produção de NO em sangue total / Effects of harpagophytum procumbens (devils claw) extracts and its fractions on Cox-1 and Cox-2 activity and nitric oxide production in whole blood.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

OBJECTIVE: The present study evaluated the effect of H. procumbens extracts and its fractions on COX-1 and COX-2 activity and NO production in whole blood assays of volunteers and OA patients. METHODS: The COX-1 and COX-2 activity was quantified as platelet TXB2 production in blood clotting and as PGE2 production in heparinized LPS-stimulated whole blood, respectively. Total NO2 -/NO3 - was determined by Griess reaction. In vitro assays were performed through incubation of the extract and fractions with whole blood from volunteers. Controls of the inhibition of COX-1 and COX-2 activity were indomethacin and etoricoxib. Before and after treating OA lumbar spine patients with devils claw the COX-1 and COX-2 activity and NO production were evaluated in their whole blood. RESULTS: The treatment promoted clinical improvement and increase in the activity of COX-1 and basal COX-2, without LPS. The crude extract did not affect the activity of both enzymes. However, harpagoside inhibited COX-1 and COX-2 activity and NO production. CONCLUSION: Devils claw promoted clinical improvement of OA patients and harpagoside must be focus of specific studies.

ASSUNTO(S)

osteoarthritis ciclooxygenase 1 iridóides/ uso terapêutico osteoartrite nitric oxide ciclooxigenase 1 harpagophytum procumbens ciclooxygenase 2 iridoids/therapeutic use Óxido nítrico ciclooxigenase 2 harpagophytum procumbens

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