Efeitos da ingestão prolongada de lactose, apos o desmame, sobre o estomago e intestinos delgado e grosso de ratos

AUTOR(ES)

Elizete Aparecida Lomazi

DATA DE PUBLICAÇÃO

1995

RESUMO

Ninety-six weanling male Wistar rats were studied to evaluate the effects of a diet containing lactose upon stomach and small and large intestines. The animals were divided into two groups which were fed either appropriate rat chow or the same chow in which cornstarch was changed by lactose to a final proportion of 20% (w/w). The animals were weighed at the beginning of the study and weekly. In 48 animals from each group, the gastric emptying of fluid was studied by measuring the gastric retention offour test meals containing lactose (5% and 10% w/v) and glucose plus galactose (5% and 10% w/v). Subsequently, the stomach, and the small and large intestines of 12 animals from each group were isolated and their wet weights determined. The length of the small intestine and the volume of the large intestine were also measured. Homogenates of the small intestine were assayed for lactase activity. Histological sections of the duodenum, the proximal and middle small intestine and the distal ileum were scanned, and the following morphometric parameters quantified: thickness of the serosal plus muscular layer, height ofthe villus, depth ofthe crypt and size ofthe epithelial cells. The body weights of both groups of animaIs were similar until the 14th day. From the 21 st day onwards the body weight of those animaIs that fed chow without lélctose was significantly greater (p<0.05). The relative fresh weight of the stomach was significantly greater in the chow plus lactose group.The values for gastric retentión obtained 15 minutes after an orogastric infusion of the solutions, were similar in both groups for the solutions containing 5% and 10% lactose and for the 10% glucose plus galactose solution. However, for the 5% glucose plus galactose solution, the value of gastric retention was lower in the chow plus lactose group (p<0.05). There was a significant increase (p<0.05) in the relative length (cm/l00g of body weight) and fresh weight (g/100g of body weight) of the small intestine in the chow plus lactose group. Morphometric analysis showed that the height of the villus in terminal ileum was significantly greater (p<0.05) in this group. The lactase specific activity of the small intestine was greater in the chow plus lactose group but this difference was not statistically significant. There were significant differences (p

ASSUNTO(S)

esvaziamento gastrico dissacaridases intestinos

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