Efeitos da L-arginina sobre as concentraÃÃes de metabÃlitos in vivo no sangue e em cicatriz de retalho miocutÃneo, e sobre a pressÃo de rotura de cicatriz cirÃrgica intestinal em ratos / Effects of L-arginine upon the concentration of metabolites, in vivo, in the blood, miocutaneous skin graft scar and on the pressure of rupture of intestinal surgical scar in rats

AUTOR(ES)
DATA DE PUBLICAÇÃO

2002

RESUMO

Cicatrization, contraction and deposition of connective tissue matrix constitute healing mechanisms for the development of the wound scar. Studies have demonstrated that L-arginine favors cicatrization, increasing the synthesis of collagen. The aim of this paper was to study the effects of L-arginine upon the concentrations of glucose, pyruvate, lactate and ketone bodies, in vivo, in blood, miocutaneous skin graft scar and on the pressure of rupture of intestinal surgical scar in rats. Forty-eight white Wistar male rats, were distributed in 2 groups: (G1/control) and G2/experiment). A pedunculated miocutaneous skin graft was raised on the dorsum of all rats followed by immediate suturing with nylon 5-0. Jejunotomies were also performed on the antimesenteric border of the small intestine. Bowel sutures were carried out utilizing synthetic material (poliglecaprone 25). Oral supplementation of L-arginine (5% of TCI/day) was delivered via gavage to G-2/Experiment rats beginning 12 hours following surgical procedures daily. G-1/Control animals were fed sodium caseinate instead. Blood samples were collected 7, 14 and 30 days later. Measurement of intestinal rupture pressure was carried out at the same intervals, following blood samples collection. Decreased concentrations of pyruvate lactate and ketone bodies in the 14th postoperative day suggest greater utilization by peripheral tissues due to a possible anabolic effect of L-arginine. Increased ketone bodies concentrations in the miocutaneous graft in the 30th postoperative day in L-arginine treated rats may be due to increased uptake of these metabolites by healing tissues. Group G-2/E intestinal rupture pressure was greater than G-1/C on the 14th day. These results suggest that oral supplementation of L-arginine has effects upon blood concentrations of metabolites and on the cutaneous, muscular and small intestine cicatrization

ASSUNTO(S)

cicatrizaÃÃo metabolism wound healing cirurgia surgical injury cicatriz - metabolismo arginina - metabolismo metabolismo cicatrizaÃÃo de feridas l-arginina trauma cirÃrgico ratos l-arginine,rats

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