Efeitos do extrato hidroalcoolico da Acanthospermun hispidum sobre a resposta imunologia

AUTOR(ES)
DATA DE PUBLICAÇÃO

2001

RESUMO

The objective of this work was to investigate the effects of A.hispidum hidroalcoholic extract (A. hispidum HAE) on the growth and differentiation of granulocyte-macrophage hematopoietic progenitor cells (CFU-GM) ITomthe bone marrow and spleen of mice infected with L. monocytogenes. In this study, groups of Balb/c mice were treated orally for 5 days with 100, 500 or 1000mg/kgl day of A. hispidum HAE. At the end of the treatment, mice were infected intraperitoneaIly with a subletal dose of L. monocytogenes (lxl05 bacteria/animaI) and sacrificed 48 or 72 hours after infection. The administration of the 3 different doses of A. hispidum HAE to normal mice led to a dose-dependent decrease in the number ofCFU-GM in the bone marrow in relation to controI. A decrease in the number of CFU-GM in the bone marrow of infected mice at 48 (I-48h) and 72 (I-72h) hours after infection was observed, when compared with controI. The effect observed in bone marrow CFU-GM number of infected mice was mantainned when mice were pre-treated with the 3 different doses of A. hispidum HAE, however the decrease was more accentuated at 72 hours after infection. Treatment with these 3 doses of A. hispidum HAE enhanced the number of CFU-GM in the spleen of normal mice, in relations to controls. A significant increase of splenic CFU-GM was also observed in infected mice. At 48 hours after the infection, the animais previously treated with the 3 different doses of A. hispidum HAE kept the number of CFU-GM at the infected (I-48h) leveI. At 72 hours, the groups treated/infected mice with the doses of 100 and 500mg/kg after infection had a decrease in the number of CFU-GM in relation to infected group (I-72h), while, infected mice pre-treated with 1000mg/kg showed an increase in the number of splenic CFU-Gmover the leves of infection. Spleen weight also increased in the groups of 500 and 1000mglkg of A. hispidum HAE in relation to controI. The infection also increased the spleen weight at both periods after infection. A decrease was observed in groups with the 3 doses of A. hispidum HAE at 48 hours after infection, whereas at 72 hours, no changes were produced in the spleen weight of infected mice when mice were pre-treated with the 3 doses. We ana1yzed the mice resistance previously treated with the 3 different doses of A. hispidum HAE and infected with a lethal dose of L. monocytogenes (3xl05 bacteriaJanimal) and observed during 30 days after infection. Thirty percent of the 1O0mglkgtreated mice and 60% of 500mglkg and 1000 mglkg treated mice survived , while 100% of control mice died into 6 days. Based on these findings, we suggest that A. hispidum HAE may induce mobilization of hematopoietic precursors .ITomthe bon~ marrow to the spleen, thus increasing the resistance of L. monocytogenes infected mice

ASSUNTO(S)

plantas medicinais listeria sistema imune

ACESSO AO ARTIGO

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